Journal: The Journal of Reproduction and Development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Expression levels of FABP4 in sheep endometrium. (a) FABP4 mRNA expression in sheep endometrium on day 4 and day 15. (b, c) FABP4 protein expression in sheep endometrium on day 4 and 15. The optical density was normalized to the density of β-actin in the same lane. (d, e) Rabbit IgG group was used as the negative control for analyzing the localization and expression of FABP4 in sheep endometrium. Data are presented as mean ± standard error with significant differences at P < 0.05 and extremely significant differences at P < 0.01. * indicates P < 0.05, ** indicates P < 0.01, and no sign indicates that the difference is not significant. The technique was repeated thrice.

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Expressing, Negative Control

Journal: The Journal of Reproduction and Development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Expression of FABP4 in SEECs. (a) Isolation and purification of SEECs (250 ×). When the cells reached the sixth passage, they became larger and rounder and began to senesce. (b) Immunofluorescence identification of SEECs (CK18) (100 ×). (c) Localization of FABP4 in SEECs (Yellow arrows are FABP4 in the nuclei) (25 ×).

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Expressing, Isolation, Purification, Immunofluorescence

Journal: The Journal of Reproduction and Development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: Hormone treatment of SEECs and detection of changes in FABP4 expression. (a) Protein expression levels of PGR and ER after combined hormone treatment. (b) The mRNA expression levels of ISG15, HOXA10, CXCL10, and RSAD2 after hormone treatment. (c) Levels of the prostaglandin secreted from the SEECs after hormone treatment.(d) Expression levels of FABP4 after hormone treatment. All data are presented as mean ± standard error. Differences were considered significant at P < 0.05 and extremely significant at P < 0.01.

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Expressing

Journal: The Journal of Reproduction and Development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: FABP4 inhibition impedes SEEC function. (a, b) Mobility of SEECs at 0, 24, 48, 72 and 96 h was measured using a scratch test. Migratory capacity was calculated as a percentage of healing area relative to time 0. (c) CCK-8 viable cell counts quantified the proliferative capacity of SEECs treated with hormone and FABP4 inhibitor BMS309403. (d–g) Expression levels of EMT after hormone and inhibitor treatment (E-cadherin, N-cadherin, Vim, and β-catenin). (h, i) Changes in endoplasmic reticulum stress-related protein CHOP and GRP78 were measured. (j, k) Changes in autophagy-related proteins p-mTOR, LC3B II/I, and P62. Data are expressed as mean ± standard error. Differences were considered significant at P < 0.05 and extremely significant at P < 0.01.

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Inhibition, CCK-8 Assay, Expressing

Journal: The Journal of Reproduction and Development

Article Title: FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation

doi: 10.1262/jrd.2023-015

Figure Lengend Snippet: TG and 3-MA treatment partially restores SEEC function after BMS30940 suppression of FABP4. (a, b) Expression levels of key proteins in the endoplasmic reticulum stress signaling pathway after combined treatment with hormones, BMS309403, and TG. (c, d) Expression levels of key proteins in autophagy and apoptosis signaling pathways after combined treatment with hormones, BMS309403, and 3-MA. (e) Secreted prostaglandin levels from SEECs after combined treatment with hormones, BMS309403, and TG or 3-MA. Data are expressed as mean ± standard error. Differences were considered significant at P < 0.05 and extremely significant at P < 0.01.

Article Snippet: SEECs were fixed with 4% paraformaldehyde at room temperature, blocked with BSA for 30 min, and FABP4 rabbit polyclonal antibody (Boster, BM4029, 1:50) and CK18 mouse monoclonal antibody (Abcam, Cambridge, UK, ab668, 1:50) were added overnight at 4°C.

Techniques: Expressing, Protein-Protein interactions

Journal: Disease Markers

Article Title: Correlation between Serum Fatty Acid Binding Protein 4 (FABP4) Levels and Cardiac Function in Patients with Thalassemia Major

doi: 10.1155/2021/5130628

Figure Lengend Snippet: Characteristics in total patients and stratified by median of fatty acid-binding protein 4 (FABP4) levels (median = 21.5 ng/mL).

Article Snippet: The serum concentration of FABP4 was quantified using a commercially available enzyme-linked immunosorbent (ELISA) assay kit for FABP4 (CSB-E12995h; Cusabio, Wuhan, China).

Techniques:

Journal: Disease Markers

Article Title: Correlation between Serum Fatty Acid Binding Protein 4 (FABP4) Levels and Cardiac Function in Patients with Thalassemia Major

doi: 10.1155/2021/5130628

Figure Lengend Snippet: Serum fatty acid-binding protein 4 (FABP4) levels in patients with thalassemia major with and without siderosis (serum ferritin > 2500 ng/mL and ≤2500 ng/mL, respectively, p = 0.003).

Article Snippet: The serum concentration of FABP4 was quantified using a commercially available enzyme-linked immunosorbent (ELISA) assay kit for FABP4 (CSB-E12995h; Cusabio, Wuhan, China).

Techniques: Binding Assay

Journal: Disease Markers

Article Title: Correlation between Serum Fatty Acid Binding Protein 4 (FABP4) Levels and Cardiac Function in Patients with Thalassemia Major

doi: 10.1155/2021/5130628

Figure Lengend Snippet: Liver enzyme and cardiac function parameters stratified by median of FABP4 levels (median = 21.5 ng/mL).

Article Snippet: The serum concentration of FABP4 was quantified using a commercially available enzyme-linked immunosorbent (ELISA) assay kit for FABP4 (CSB-E12995h; Cusabio, Wuhan, China).

Techniques: Blocking Assay

Journal: Disease Markers

Article Title: Correlation between Serum Fatty Acid Binding Protein 4 (FABP4) Levels and Cardiac Function in Patients with Thalassemia Major

doi: 10.1155/2021/5130628

Figure Lengend Snippet: Correlation between serum ferritin and FABP4.

Article Snippet: The serum concentration of FABP4 was quantified using a commercially available enzyme-linked immunosorbent (ELISA) assay kit for FABP4 (CSB-E12995h; Cusabio, Wuhan, China).

Techniques:

Journal: Disease Markers

Article Title: Correlation between Serum Fatty Acid Binding Protein 4 (FABP4) Levels and Cardiac Function in Patients with Thalassemia Major

doi: 10.1155/2021/5130628

Figure Lengend Snippet: Correlation between left atrial volume index (LAVI) and FABP4.

Article Snippet: The serum concentration of FABP4 was quantified using a commercially available enzyme-linked immunosorbent (ELISA) assay kit for FABP4 (CSB-E12995h; Cusabio, Wuhan, China).

Techniques:

Journal: Endocrinology

Article Title: In utero exposure to the endocrine disruptor di-(2-ethylhexyl) phthalate induces long-term changes in gene expression in the adult male adrenal gland.

doi: 10.1210/en.2013-1921

Figure Lengend Snippet: Figure 6. Serum markers that correlate with in utero exposure to DEHP. A, Heatmap of differentially expressed genes at PND21 and PND60 in offspring of rats exposed in utero to 100- or 300-mg DEHP/kgd was constructed to identify genes altered at both time points and doses. Selected gene targets (bold) were used to evaluate putative serum markers. Serum levels of AQP7 (B), FABP4 (C), and PCK1 (D) in adult rats exposed in utero to the indicated doses of DEHP. Data in C and D are means SD (n 4); **, ANOVA, P .01. E, Relationship between FABP4 and PCK1 adult rat serum levels to in utero DEHP exposure doses used. FABP4, correlation coefficient 0.37, P .49. PCK1, correlation coefficient 0.77, P .1.

Article Snippet: ELISAs were used to quantify aquaporin 7 (AQP7) (catalog number CSB-EL001967RA; Cusabio Biotech, Cedarlane Laboratories), fatty acid-binding protein 4 (FABP4) (catalog number CSB-E13027r; Cusabio Biotech), and phosphoenolpyruvate carboxykinase I (PCK1) (catalog number sE90936Ra; USCN Life Science, Inc, Cedarlane Laboratories), according to the instructions of each manufacturer.

Techniques: In Utero, Construct

Journal: Histochemistry and Cell Biology

Article Title: Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae

doi: 10.1007/s00418-020-01953-y

Figure Lengend Snippet: FABP4-immnopositive cells in the chondro-osseous junction (COJ) of WT and FABP5 −/− mice. Light micrograph ( a ) and 3D-reconstructed image ( b , c ) of FABP5-immunopositive septoclasts in WT mice. Light micrograph ( d ) and 3D-reconstructed image ( e , f ) of FABP4-immunopositive cells in WT mice. Light micrograph ( g ) and 3D-reconstructed image ( h , i ) of FABP4-immunopositive cells in FABP5 −/− mice. d , g Arrowheads: FABP4 immunopositive cells. c , f , i Images drawing the outline of septoclasts as same as ( b , e and h ), respectively. Asterisks: cell bodies of septoclasts, Dotted lines: boundaries of septoclastic processes, Solid lines: outline of septoclastic body and processes, P length of process. j Mean SD, [ n = 16]. NS no significance. Scale bars: 100 μm ( a , d , g ), 6.1 μm (length of the side of a square in 3D grid, b , c , e , f , h , i )

Article Snippet: For double immunofluorescent staining, sections were treated overnight at room temperature with a mixture of rabbit anti-FABP4 (12,802–1-AP; 1:50, Proteintech, Chicago, IL, USA) and goat anti-mouse CD31 (AF3628; 10 μg/ml; R&D system), or rabbit anti-FABP4 (12,802–1-AP; 1:50, Proteintech) and goat anti-mouse PDGFRβ (AF1042; 10 μg/ml; R&D system), or rabbit anti-FABP4 (12,802–1-AP; 1:50, Proteintech) and goat anti-human cathepsin B (AF965; 10 μg/ml, R&D Systems), or rabbit anti-rat FABP5 (0.5 μg/ml, Owada et al. ) and goat anti-mouse FABP4 (AF1443; 10 μg/ml, R&D Systems), or rabbit anti-human PPARγ (MA5-14,889; 1:50, Invitrogen, Carlsbad, CA, USA) and goat anti-mouse FABP4 (AF1443; 10 μg/ml, R&D Systems).

Techniques:

Journal: Histochemistry and Cell Biology

Article Title: Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae

doi: 10.1007/s00418-020-01953-y

Figure Lengend Snippet: Expression of FABP4 in FABP5-immunopositive septoclasts in WT mice. Light micrographs of longitudinal sections at the chondro-osseous junction (COJ) of proximal tibiae of WT mice stained for FABP4 (red) plus cathepsin B (green) ( a ), for FABP4 (red) plus FABP5 (green) ( b ), for FABP4 (red) plus PDGFRβ (green) ( c ), and for FABP4 (red) plus CD31 (green) ( d ). Scale bars: 20 μm

Article Snippet: For double immunofluorescent staining, sections were treated overnight at room temperature with a mixture of rabbit anti-FABP4 (12,802–1-AP; 1:50, Proteintech, Chicago, IL, USA) and goat anti-mouse CD31 (AF3628; 10 μg/ml; R&D system), or rabbit anti-FABP4 (12,802–1-AP; 1:50, Proteintech) and goat anti-mouse PDGFRβ (AF1042; 10 μg/ml; R&D system), or rabbit anti-FABP4 (12,802–1-AP; 1:50, Proteintech) and goat anti-human cathepsin B (AF965; 10 μg/ml, R&D Systems), or rabbit anti-rat FABP5 (0.5 μg/ml, Owada et al. ) and goat anti-mouse FABP4 (AF1443; 10 μg/ml, R&D Systems), or rabbit anti-human PPARγ (MA5-14,889; 1:50, Invitrogen, Carlsbad, CA, USA) and goat anti-mouse FABP4 (AF1443; 10 μg/ml, R&D Systems).

Techniques: Expressing, Staining

Journal: Histochemistry and Cell Biology

Article Title: Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae

doi: 10.1007/s00418-020-01953-y

Figure Lengend Snippet: Cell count of FABP4-immunopositive septoclasts ( a ) and light micrographs of longitudinal sections stained for PPAR (red) plus FABP4 (green) with DAPI (blue) at the COJ of proximal tibiae of WT mice ( b – d ) and FABP5 −/− mice ( e – g ). Number of FABP5-immunopositive cells represents that of septoclasts ( a ). Arrowheads: lack ( b – d ) or occurrence ( e – g ) of immunoreactivity of PPAR in the nucleus of FABP4-positive septoclasts. a Mean SD, * P < 0.001 [ n = 20]. Scale bars: 20 μm

Article Snippet: For double immunofluorescent staining, sections were treated overnight at room temperature with a mixture of rabbit anti-FABP4 (12,802–1-AP; 1:50, Proteintech, Chicago, IL, USA) and goat anti-mouse CD31 (AF3628; 10 μg/ml; R&D system), or rabbit anti-FABP4 (12,802–1-AP; 1:50, Proteintech) and goat anti-mouse PDGFRβ (AF1042; 10 μg/ml; R&D system), or rabbit anti-FABP4 (12,802–1-AP; 1:50, Proteintech) and goat anti-human cathepsin B (AF965; 10 μg/ml, R&D Systems), or rabbit anti-rat FABP5 (0.5 μg/ml, Owada et al. ) and goat anti-mouse FABP4 (AF1443; 10 μg/ml, R&D Systems), or rabbit anti-human PPARγ (MA5-14,889; 1:50, Invitrogen, Carlsbad, CA, USA) and goat anti-mouse FABP4 (AF1443; 10 μg/ml, R&D Systems).

Techniques: Cell Counting, Staining

Journal: Histochemistry and Cell Biology

Article Title: Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae

doi: 10.1007/s00418-020-01953-y

Figure Lengend Snippet: Cell count of FABP4-immunopositive septoclasts ( a ) and light micrographs of longitudinal sections stained for PPAR (red) plus FABP4 (green) with DAPI (blue) at the COJ of proximal tibiae of control mice ( b – d ) and PPAR agonist (GW1929)-treated mice ( e – g ). Number of FABP5-immunopositive cells represents the number of septoclasts ( a ). Arrowheads: lack ( b – d ) or occurrence ( e – g ) of immunoreactivity of PPAR in the nucleus of FABP4-positive septoclasts. a Mean SD, * P < 0.01 [ n = 20]. Scale bars: 20 μm

Article Snippet: For double immunofluorescent staining, sections were treated overnight at room temperature with a mixture of rabbit anti-FABP4 (12,802–1-AP; 1:50, Proteintech, Chicago, IL, USA) and goat anti-mouse CD31 (AF3628; 10 μg/ml; R&D system), or rabbit anti-FABP4 (12,802–1-AP; 1:50, Proteintech) and goat anti-mouse PDGFRβ (AF1042; 10 μg/ml; R&D system), or rabbit anti-FABP4 (12,802–1-AP; 1:50, Proteintech) and goat anti-human cathepsin B (AF965; 10 μg/ml, R&D Systems), or rabbit anti-rat FABP5 (0.5 μg/ml, Owada et al. ) and goat anti-mouse FABP4 (AF1443; 10 μg/ml, R&D Systems), or rabbit anti-human PPARγ (MA5-14,889; 1:50, Invitrogen, Carlsbad, CA, USA) and goat anti-mouse FABP4 (AF1443; 10 μg/ml, R&D Systems).

Techniques: Cell Counting, Staining

Journal: Biomedicines

Article Title: Fatty Acid Binding Protein 4 Could Be a Linking Biomarker Between Periodontitis and Systemic Diseases

doi: 10.3390/biomedicines13020402

Figure Lengend Snippet: Baseline demographic and clinical characteristics according to periodontal stage.

Article Snippet: The blood sample was centrifuged in the laboratory to separate the serum, and then confirmed the P. gingivalis antibody titer by P. gingivalis antibody titer ELISA kit (Boster Biological Technology, New York, NY, USA) and FABP4 levels using Human FABP4/A Fabp ELISA Kit (PicoKine ® , Pleasanton, CA, USA).

Techniques:

Journal: Biomedicines

Article Title: Fatty Acid Binding Protein 4 Could Be a Linking Biomarker Between Periodontitis and Systemic Diseases

doi: 10.3390/biomedicines13020402

Figure Lengend Snippet: (A) Comparison of indicators before and after non-surgical periodontal treatment. (B) Comparison of indicators before and after periodontal treatment, catagorized by stage.

Article Snippet: The blood sample was centrifuged in the laboratory to separate the serum, and then confirmed the P. gingivalis antibody titer by P. gingivalis antibody titer ELISA kit (Boster Biological Technology, New York, NY, USA) and FABP4 levels using Human FABP4/A Fabp ELISA Kit (PicoKine ® , Pleasanton, CA, USA).

Techniques: Comparison

Journal: Biomedicines

Article Title: Fatty Acid Binding Protein 4 Could Be a Linking Biomarker Between Periodontitis and Systemic Diseases

doi: 10.3390/biomedicines13020402

Figure Lengend Snippet: ( A – C ) FABP4 changes after periodontal treatment in each stage. ( D – F ) P. gingivalis antibody titer level changes after periodontal treatment in each stage.

Article Snippet: The blood sample was centrifuged in the laboratory to separate the serum, and then confirmed the P. gingivalis antibody titer by P. gingivalis antibody titer ELISA kit (Boster Biological Technology, New York, NY, USA) and FABP4 levels using Human FABP4/A Fabp ELISA Kit (PicoKine ® , Pleasanton, CA, USA).

Techniques:

Journal: Biomedicines

Article Title: Fatty Acid Binding Protein 4 Could Be a Linking Biomarker Between Periodontitis and Systemic Diseases

doi: 10.3390/biomedicines13020402

Figure Lengend Snippet: ( A – D ) Correlations between changes in FABP4 and Clinical parameters. ( E ) Correlations between changes in FABP4 and P. gingivalis antibody titer.

Article Snippet: The blood sample was centrifuged in the laboratory to separate the serum, and then confirmed the P. gingivalis antibody titer by P. gingivalis antibody titer ELISA kit (Boster Biological Technology, New York, NY, USA) and FABP4 levels using Human FABP4/A Fabp ELISA Kit (PicoKine ® , Pleasanton, CA, USA).

Techniques: